Laboratory of Physical Molecular Biology (chromatin fiber imaging)

The Laboratory of Physical Molecular Biology uses an interdisciplinary approach combining the disciplines of molecular biology, biochemistry, engineering, and physics to attempt to understand at the single nucleosome and single chromatin fiber level how chromatin structure and dynamics regulate biological processes that use DNA as a template.  Single-molecule techniques provide the sensitivity to detect, image, and elucidate small, yet physiologically relevant, changes in chromatin structure and dynamics. 

The Laboratory is applying several single-molecule approaches such as atomic force microscopy (AFM), magnetic tweezers, optical tweezers, and single-pair fluorescence resonance energy transfer (spFRET) to native or reconstituted chromatin fibers of various protein compositions.

Dr. Leuba's Research web site